Douglas Guzior

Douglas Guzior

Gut Microbiologist, Biochemist, and Data Scientist with expertise in multi-omics and host-microbiome interactions.

Publications

You can also find my articles on my Google Scholar profile.

Longitudinal analyses of infants’ microbiome and metabolome reveal microbes and metabolites with seemingly coordinated dynamics

Published in Communications Biology, 2024

Uncovering the intricate connections between the development of the infant gut microbiome and corresponding shifts in fecal metabolite profiles, revealing dynamic interplay that may influence infant health. Read more

Recommended citation: Wu H, Guzior DV, Martin C, Neugebauer KA, Rzepka MM, Lumeng JC, Quinn RA, de los Campos G. 2024. Longitudinal analyses of infants microbiome and metabolome reveal microbes and metabolites with seemingly coordinated dynamics. Commun Biol 7:1506. https://doi.org/10.1038/s42003-024-07015-6.

Shifts in the functional capacity and metabolite composition of the gut microbiome during recovery from enteric infection

Published in Frontiers in Cellular and Infection Microbiology, 2024

Investigating the impact of enteric infection on the gut microbiome uncovers higher metabolic capacity during infection, yet greater chemical diversity afterwards. Read more

Recommended citation: Hansen ZA, Schilmiller AL, Guzior DV, Rudrik JT, Quinn RA, Vasco KA and Manning SD. 2024. Shifts in the functional capacity and metabolite composition of the gut microbiome during recovery from enteric infection. . https://doi.org/10.3389/fcimb.2024.1359576

Bile salt hydrolase acyltransferase activity expands bile acid diversity

Published in Nature, 2024

Bile salt hydrolase, an enzyme with decades of research behind it, is identified as one of the culprits behind microbial bile acid conjugation, diversifying the bile acid pool and further establishing connections between microbially conjugated bile acids and gastrointestinal physiology. Read more

Recommended citation: Guzior DV, Okros M, Shivel M, Armwald B, Bridges C, Fu Y, Martin C, Schilmiller A, Miller WM, Ziegler KM, Sims MD, Maddens ME, Graham SF, Hausinger RP, Quinn RA. 2024. Bile salt hydrolase acyltransferase activity expands bile acid diversity. Nature 626:852-858. https://doi.org/10.1038/s41586-024-07017-8

Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice

Published in Frontiers in Cell and Developmental Biology, 2024

Dietary prune supplementation, even at 5%, can mitigate glucocorticoid-induced bone loss and shift gut microbiota in a murine model. Greater supplementation significantly enhanced bone density and influenced gut microbiota changes associated with improved bone health in mice. Read more

Recommended citation: Chargo NJ, Neugebauer K, Guzior DV, Quinn RA, Parameswaran N, McCabe LR. 2024. Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice. Front. Cell Dev. Biol. 11:1324649. https://doi.org/10.3389/fcell.2023.1324649

Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy

Published in Respiratory Research, 2023

Investigation into prolonged effects of ETI therapy on patients with cystic fibroses showed enhanced lung function and more varied lung microbiomes over time, even though colonization with CF pathogens are still prevalent. Read more

Recommended citation: Martin C, Guzior DV, Gonzalez CT, Okros M, Mielke J, Padillo L, Thomas R, McClelland M, Conrad D, Widder S, Quinn RA. 2023. Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy. Respir. Res. 24:317. https://doi.org/10.1186/s12931-023-02630-z

A multi-omic trip through the human gut

Published in Nature Metabolism, 2023

Most of human microbiome research has focused on analysing faecal samples, which represent the final stop of the digestive journey. Two recent articles use a novel sampling approach to capture luminal content at different points during digestion and reveal that the analysis of faecal samples tells only a fraction of the story. Read more

Recommended citation: Quinn RA, Martin C, Guzior DV. 2023. A multi-omic trip through the human gut. Nat Metab 5(5): 720–721. https://doi.org/10.1038/s42255-023-00773-3

Baat gene knockout alters early life development and the gut microbiome and reveals unusual bile acids in mice

Published in Journal of Lipid Research, 2022

Bile acid conjugation is important for early life development of mice, as knocking out the gene responsible for canonical murine bile acid conjugation results in significant physiological deficits early in life. Read more

Recommended citation: Neugebauer KA, Okros M, Guzior DV, Feiner J, Chargo NJ, Rzepka M, Schilmiller AL, O’Reilly S, Jones AD, Watson VE, Luyendyk JP, McCabe L, Quinn RA. 2022. Baat gene knockout alters early life development and the gut microbiome and reveals unusual bile acids in mice. J Lipid Res 63(12):100297. https://doi.org/10.1016/j.jlr.2022.100297

Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection

Published in The ISME Journal, 2022

Changes in the cystic fibrosis lung can be predicted utilizing in silico models and further validated with experiments in vitro. Read more

Recommended citation: Ghuneim LAJ, Raghuvanshi R, Neugebauer KA, Guzior DV, Martin H. C, Schena B, Feiner JM, Castillo-Bahena A, Mielke J, McClelland M, Conrad D, Klapper I, Zhang T, Quinn RA. 2022. Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection. ISME J 16(9):2065–2075. https://doi.org/10.1038/s41396-022-01252-5

Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis

Published in Cell Reports, 2021

Further understanding the impacts of antibiotics on the cecal microbiome and metabolome in the context of hyperglycemia. Read more

Recommended citation: Wurster JI, Peterson RL, Brown CE, Penumutchu S, Guzior DV, Neugebauer K, Sano WH, Sebastian MM, Quinn RA, Belenky P. 2021. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis. Cell Rep 37(11):110113. https://doi.org/10.1016/j.celrep.2021.110113

A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung

Published in Journal of Cystic Fibrosis, 2021

The first description of how Elexacaftor-Tezacaftor-Ivacaftor, also known as TRIKAFTA, therapy alters the lung microbiome community structure of patients with cystic fibrosis. Read more

Recommended citation: Sosinski LM, H CM, Neugebauer KA, Ghuneim L-AJ, Guzior DV, Castillo-Bahena A, Mielke J, Thomas R, McClelland M, Conrad D, Quinn RA. 2021. A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung. J Cyst Fibros 21(6):996-1005. https://doi.org/10.1016/j.jcf.2021.11.003

Investigation of synovial fluid induced Staphylococcus aureus aggregate development and its impact on surface attachment and biofilm formation

Published in PLoS ONE, 2020

This paper describes how various components found within synovial fluid, the biological lubricant present in joints, can impact aggregation and surface attachment during infection. Read more

Recommended citation: Pestrak MJ, Gupta TT, Dusane DH, Guzior DV, Staats A, Harro J, Horswill AR, Stoodley P. 2020. Investigation of synovial fluid induced *Staphylococcus aureus* aggregate development and its impact on surface attachment and biofilm formation. PLOS One 15(4):e0231791. https://doi.org/10.1371/journal.pone.0231791