Douglas Guzior

Douglas Guzior

Gut Microbiologist, Biochemist, and Data Scientist with expertise in multi-omics and host-microbiome interactions.

Publications

You can also find my articles on my Google Scholar profile.

Evexomostat (SDX-7320), a methionine aminopeptidase type 2 inhibitor, stimulates weight loss and inhibits obesity-accelerated tumor growth

Published in Frontiers in Oncology, 2026

The polymer-conjugated METAP2 inhibitor SDX-7320 promotes weight loss, improves insulin sensitivity, and attenuates obesity-accelerated tumor growth in mice, outperforming tirzepatide in tumor growth inhibition despite causing less weight loss. Read more

Recommended citation: Cornelius P, Mayes BA, Dannenberg AJ, Dufour PJ, Little S, Guzior DV, Petersen JS, Shanahan JM, Carver BJ. 2026. Evexomostat (SDX-7320), a methionine aminopeptidase type 2 inhibitor, stimulates weight loss and inhibits obesity-accelerated tumor growth. Front Oncol 16:1751681. https://doi.org/10.3389/fonc.2026.1751681

Serum cholic acid and cecal Faecalibaculum increase in a male-specific manner in a murine hepatocellular carcinoma model

Published in Journal of Lipid Research, 2025

In a bile acid excess model of hepatocellular carcinoma, tumor-bearing males accumulate free cholic acid in serum alongside sex-specific gut microbiota shifts, with unconjugated cholic acid tracking the abundance of Faecalibaculum. Read more

Recommended citation: Dean AE, Guzior DV, Quinn RA, Gaulke CA, Anakk S. 2025. Serum cholic acid and cecal *Faecalibaculum* increase in a male-specific manner in a murine hepatocellular carcinoma model. J Lipid Res 67(1):100954. https://doi.org/10.1016/j.jlr.2025.100954

Short-term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice

Published in Physiological Reports, 2025

Just 10 days of methionine restriction, even while maintaining a high fat diet, rapidly reverses metabolic dysfunction in mice and produces distinct proteomic and metabolomic signatures across adipose tissue, liver, aorta, and plasma. Read more

Recommended citation: McGilvrey MI, Fortier B, Cooke D, Mahdi MA, Tero B, Potts CM, Kaija A, Ryzhova L, Cora C, Richardson A, Guzior DV, Pinz I, Vary C, Koza RA, Ables G, Liaw L. 2025. Short-term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice. Physiol Rep 13(12):e70405. https://doi.org/10.14814/phy2.70405

Microenvironmental effects of a non-antibiotic therapy for a chronic polymicrobial infection alter microbial physiology, competition, and virulence

Published in The ISME Journal, 2025

Modeling the effects of Elexacaftor-Tezacaftor-Ivacaftor therapy on airway mucus shows that reduced nutrient availability dampens Pseudomonas aeruginosa virulence factor production, reducing competition and toxicity in cystic fibrosis lung communities. Read more

Recommended citation: González CT, Martin C, Crane M, Gutierrez K, Thomas J, Remisoski L, Okros M, Fu Y, Guzior DV, Finkhouse D, Bridges C, Mielke J, Querido G, Padillo L, Girgis R, McClelland M, Conrad D, Li X, Quinn RA. 2025. Microenvironmental effects of a non-antibiotic therapy for a chronic polymicrobial infection alter microbial physiology, competition, and virulence. ISME J 19:wraf125. https://doi.org/10.1093/ismejo/wraf125

Balance between bile acid conjugation and hydrolysis activity can alter outcomes of gut inflammation

Published in Nature Communications, 2025

Host-conjugated bile acids protect against colitis in Baat knockout mice, but their benefit depends on a delicate balance with microbial bile salt hydrolase activity, with specific bsh genes enriched in people with Crohn’s disease. Read more

Recommended citation: Fu Y, Guzior DV, Okros M, Bridges C, Rosset SL, González CT, Martin C, Karunarathne H, Watson VE, Quinn RA. 2025. Balance between bile acid conjugation and hydrolysis activity can alter outcomes of gut inflammation. Nat Commun 16:3434. https://doi.org/10.1038/s41467-025-58649-x

Host metabolism balances microbial regulation of bile acid signalling

Published in Nature, 2025

A family of host-produced bile acid-methylcysteamine conjugates, made by the intestinal enzyme VNN1, act as potent FXR antagonists that counterbalance the signalling of microbiota-derived free bile acids. Read more

Recommended citation: Won TH, Arifuzzaman M, Parkhurst CN, Miranda IC, Zhang B, Hu E, Kashyap S, Letourneau J, Jin WB, Fu Y, Guzior DV, Quinn RA, Guo CJ, David LA, Artis D, Schroeder FC. 2025. Host metabolism balances microbial regulation of bile acid signalling. Nature 638(8049):216–224. https://doi.org/10.1038/s41586-024-08379-9

Longitudinal analyses of infants’ microbiome and metabolome reveal microbes and metabolites with seemingly coordinated dynamics

Published in Communications Biology, 2024

Uncovering the intricate connections between the development of the infant gut microbiome and corresponding shifts in fecal metabolite profiles, revealing dynamic interplay that may influence infant health. Read more

Recommended citation: Wu H, Guzior DV, Martin C, Neugebauer KA, Rzepka MM, Lumeng JC, Quinn RA, de los Campos G. 2024. Longitudinal analyses of infants microbiome and metabolome reveal microbes and metabolites with seemingly coordinated dynamics. Commun Biol 7:1506. https://doi.org/10.1038/s42003-024-07015-6

Shifts in the functional capacity and metabolite composition of the gut microbiome during recovery from enteric infection

Published in Frontiers in Cellular and Infection Microbiology, 2024

Investigating the impact of enteric infection on the gut microbiome uncovers higher metabolic capacity during infection, yet greater chemical diversity afterwards. Read more

Recommended citation: Hansen ZA, Schilmiller AL, Guzior DV, Rudrik JT, Quinn RA, Vasco KA and Manning SD. 2024. Shifts in the functional capacity and metabolite composition of the gut microbiome during recovery from enteric infection. Front Cell Infect Microbiol 14:1359576. https://doi.org/10.3389/fcimb.2024.1359576

Bile salt hydrolase acyltransferase activity expands bile acid diversity

Published in Nature, 2024

Bile salt hydrolase, an enzyme with decades of research behind it, is identified as one of the culprits behind microbial bile acid conjugation, diversifying the bile acid pool and further establishing connections between microbially conjugated bile acids and gastrointestinal physiology. Read more

Recommended citation: Guzior DV, Okros M, Shivel M, Armwald B, Bridges C, Fu Y, Martin C, Schilmiller A, Miller WM, Ziegler KM, Sims MD, Maddens ME, Graham SF, Hausinger RP, Quinn RA. 2024. Bile salt hydrolase acyltransferase activity expands bile acid diversity. Nature 626:852-858. https://doi.org/10.1038/s41586-024-07017-8

Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice

Published in Frontiers in Cell and Developmental Biology, 2024

Dietary prune supplementation, even at 5%, can mitigate glucocorticoid-induced bone loss and shift gut microbiota in a murine model. Greater supplementation significantly enhanced bone density and influenced gut microbiota changes associated with improved bone health in mice. Read more

Recommended citation: Chargo NJ, Neugebauer K, Guzior DV, Quinn RA, Parameswaran N, McCabe LR. 2024. Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice. Front. Cell Dev. Biol. 11:1324649. https://doi.org/10.3389/fcell.2023.1324649

Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy

Published in Respiratory Research, 2023

Investigation into prolonged effects of ETI therapy on patients with cystic fibroses showed enhanced lung function and more varied lung microbiomes over time, even though colonization with CF pathogens are still prevalent. Read more

Recommended citation: Martin C, Guzior DV, Gonzalez CT, Okros M, Mielke J, Padillo L, Thomas R, McClelland M, Conrad D, Widder S, Quinn RA. 2023. Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy. Respir. Res. 24:317. https://doi.org/10.1186/s12931-023-02630-z

A multi-omic trip through the human gut

Published in Nature Metabolism, 2023

Most of human microbiome research has focused on analysing faecal samples, which represent the final stop of the digestive journey. Two recent articles use a novel sampling approach to capture luminal content at different points during digestion and reveal that the analysis of faecal samples tells only a fraction of the story. Read more

Recommended citation: Quinn RA, Martin C, Guzior DV. 2023. A multi-omic trip through the human gut. Nat Metab 5(5): 720–721. https://doi.org/10.1038/s42255-023-00773-3

Baat gene knockout alters post-natal development, the gut microbiome, and reveals unusual bile acids in mice

Published in Journal of Lipid Research, 2022

Bile acid conjugation is important for early life development of mice, as knocking out the gene responsible for canonical murine bile acid conjugation results in significant physiological deficits early in life. Read more

Recommended citation: Neugebauer KA, Okros M, Guzior DV, Feiner J, Chargo NJ, Rzepka M, Schilmiller AL, O’Reilly S, Jones AD, Watson VE, Luyendyk JP, McCabe L, Quinn RA. 2022. Baat gene knockout alters post-natal development, the gut microbiome, and reveals unusual bile acids in mice. J Lipid Res 63(12):100297. https://doi.org/10.1016/j.jlr.2022.100297

Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection

Published in The ISME Journal, 2022

Changes in the cystic fibrosis lung can be predicted utilizing in silico models and further validated with experiments in vitro. Read more

Recommended citation: Ghuneim LAJ, Raghuvanshi R, Neugebauer KA, Guzior DV, Martin C, Schena B, Feiner JM, Castillo-Bahena A, Mielke J, McClelland M, Conrad D, Klapper I, Zhang T, Quinn RA. 2022. Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection. ISME J 16(9):2065–2075. https://doi.org/10.1038/s41396-022-01252-5

Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis

Published in Cell Reports, 2021

Further understanding the impacts of antibiotics on the cecal microbiome and metabolome in the context of hyperglycemia. Read more

Recommended citation: Wurster JI, Peterson RL, Brown CE, Penumutchu S, Guzior DV, Neugebauer K, Sano WH, Sebastian MM, Quinn RA, Belenky P. 2021. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis. Cell Rep 37(11):110113. https://doi.org/10.1016/j.celrep.2021.110113

A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung

Published in Journal of Cystic Fibrosis, 2021

The first description of how Elexacaftor-Tezacaftor-Ivacaftor, also known as TRIKAFTA, therapy alters the lung microbiome community structure of patients with cystic fibrosis. Read more

Recommended citation: Sosinski LM, Martin C, Neugebauer KA, Ghuneim L-AJ, Guzior DV, Castillo-Bahena A, Mielke J, Thomas R, McClelland M, Conrad D, Quinn RA. 2021. A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung. J Cyst Fibros 21(6):996-1005. https://doi.org/10.1016/j.jcf.2021.11.003

Investigation of synovial fluid induced Staphylococcus aureus aggregate development and its impact on surface attachment and biofilm formation

Published in PLoS ONE, 2020

This paper describes how various components found within synovial fluid, the biological lubricant present in joints, can impact aggregation and surface attachment during infection. Read more

Recommended citation: Pestrak MJ, Gupta TT, Dusane DH, Guzior DV, Staats A, Harro J, Horswill AR, Stoodley P. 2020. Investigation of synovial fluid induced *Staphylococcus aureus* aggregate development and its impact on surface attachment and biofilm formation. PLOS One 15(4):e0231791. https://doi.org/10.1371/journal.pone.0231791