Curriculum Vitae
Also available to download here.
Last updated March 17th, 2024.
Education
Ph.D., Microbiology, Genetics, & Immunology
Michigan State University | East Lansing, MI | Aug 2019 - Apr 2024
Dissertation: Elucidating the mechanisms behind microbial bile acid conjugation and its impacts on host microbiota and physiology.
B.S. Microbiology, cum laude
The Ohio State University | Columbus, OH | Aug 2015 - May 2019
Thesis: Determining the impact of host-synovial fluid factors on Staphylococcus aureus aggregation.
Experience
Graduate Research Assistant | Michigan State University | East Lansing, MI | Aug 2019 - Apr 2024
Quinn Lab - Departments of Microbiology, Genetics, & Immunology and Biochemistry & Molecular Biology
My primary research project focused on determining mechanisms behind bile acid conjugation by intestinal microorganisms and investigated consequences of this aspect of microbial bile acid metabolism on the host.
- Data steward to a 1,400 sample mother-infant cohort, with paired microbiome and metabolome datasets.
- Performed enzyme kinetic experiments to characterize bile acid conjugation by bile salt hydrolase.
- Analyzed and identified key changes in the metabolome and microbiome across a variety of clinical, environmental, and in vitro microbiological sample types.
Visiting Graduate Researcher | Wellcome Sanger Institute | Cambridge, England, UK | Feb 2023 - Mar 2023
Lawley Lab - Host-Microbiota Interactions
- Taught untargeted mass spectrometry experimental design, sample preparation, and analysis techniques.
- Developed skills involved in genomic, metagenomic, and phylogenetic analyses leveraging high-performance computation.
Undergraduate Research Assistant | The Ohio State University | Columbus, OH | Oct 2018 - Jun 2019
Stoodley Lab - Department of Microbial Infection and Immunology
- Developed high-throughput methods to quantify biofilm-independent Staphylococcus aureus aggregation using 96-well plate imaging and .
- Determined relative contribution of synovial fluid components in cell aggregation within the context of periprosthetic joint infection.
Undergraduate Research Assistant | The Ohio State University | Columbus, OH | Feb 2017 - Aug 2018
Roy Lab - Center for Minimally Invasive Surgery, Department of Surgery
- Assisted with development and data collection of a maxillofacial burn wound model.
- Investigated the roles of host and bacterial extracellular vesicles in infection.
Publications
Guzior DV, Okros M, Shivel M, Armwald B, Bridges C, Fu Y, Martin C, Schilmiller AL, Miller WM, Ziegler KM, Sims MD, Maddens ME, Graham SF, Hausinger RP, Quinn RA. 2024. Bile salt hydrolase/acyltransferase activity shapes bile acid diversity. Nature 626:852–858. doi: 10.1038/s41586-024-07017-8
Chargo NJ, Neugebauer K, Guzior DV, Quinn RA, Parameswaran N, McCabe LR. 2024. Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice. Front Cell Dev Biol 11:1324649. doi: 10.3389/fcell.2023.1324649
Martin C, Guzior DV, Gonzalez CT, Okros M, Mielke J, Padillo L, Thomas R, McClelland M, Conrad D, Widder S, Quinn RA. 2023. Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy. Respir Res 24:317. doi: 10.1186/s12931-023-02630-z
Quinn RA, Martin C, Guzior DV. 2023. A multi-omic trip through the human gut. Nat Metab 5:720–721. doi: 10.1038/s42255-023-00773-3
Neugebauer KA, Okros M, Guzior DV, Feiner J, Chargo NJ, Rzepka M, Schilmiller AL, O’Reilly S, Jones AD, Watson VE, Luyendyk JP, McCabe LR, Quinn RA. 2022. Baat gene knockout alters post-natal development, the gut microbiome, and reveals unusual bile acid conjugates in mice. J Lipid Res 63:100297. doi: 10.1016/j.jlr.2022.100297
Ghuneim L-AJ, Raghuvanshi R, Neugebauer KA, Guzior DV, Martin C, Schena B, Feiner JM, Castillo-Bahena A, Mielke J, McClelland M, Conrad D, Klapper I, Zhang T, Quinn RA. 2022. Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection. ISME J 16:2065–2075. doi: 10.1038/s41396-022-01252-5
Sosinski LM, Martin C, Neugebauer KA, Ghuneim L-AJ, Guzior DV, Castillo-Bahena A, Mielke J, Thomas R, McClelland M, Conrad D, Quinn RA. 2022. A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung. J Cyst Fibros 21:996–1005. doi: 10.1016/j.jcf.2021.11.003
Wurster JI, Peterson RL, Brown CE, Penumutchu S, Guzior DV, Neugebauer KA, Sano WH, Sebastian MM, Quinn RA, Belenky P. 2021. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis. Cell Rep 37:110113. doi: 10.1016/j.celrep.2021.110113
Guzior DV, Quinn RA. 2021. Review: microbial transformations of human bile acids. Microbiome 9:140. doi: 10.1186/s40168-021-01101-1
Pestrak MJ, Gupta TT, Dusane DH, Guzior DV, Staats A, Harro J, Horswill A, Stoodley P. 2020. Investigation of synovial fluid induced Staphylococcus aureus aggregate development and its impact on surface attachment and biofilm formation. PLoS ONE 15:e0231791. doi: 10.1371/journal.pone.0231791
Conference Presentations
Talks
Guzior DV, Wu H, Martin C, Rzepka M, Neugebauer KA, Lumeng J, de los Campos G, Quinn RA. Developmental dynamics of the infant fecal metabolome.
MANA 2023 | October 2023 | Columbia, MO
Guzior DV, Quinn RA. Bile salt hydrolase aminoacyltransferase activity from gut anaerobes expands the human bile acid pool.
Anaerobe 2023 | July 2023 | Cardiff, Wales, UK | Awarded Best Offered Talk
Guzior DV, Quinn RA. Reversing Dogma: Bile Salt Hydrolase.
Anaerobe Congress | July 2022 | Seattle, WA
Posters
Guzior DV, Wu H, Martin C, Rzepka M, Neugebauer K, Lumeng J, de los Campos G, Quinn RA. Microbiome and metabolome dynamics during the first year of life.
Great Lakes Pediatric Research Day | April 2023 | Grand Rapids, MI
Guzior DV, Quinn RA. Diversity and phylogenetic relationships of bile acid conjugation within the Lachnospiraceae.
ASM Microbe | June 2022 | Washington, D.C.
Guzior DV, Quinn RA. Analyzing the diversity of in vitro microbially conjugated bile acid products.
3rd Annual Metabolomics Association of North America Conference | October 2021 | Virtual
Guzior DV, Das A, Roy S, Sen CK. Non-invasive preclinical porcine maxillofacial model to study excessive scarring of the face following burn injury.
OSUMC Research Day | April 2019 | Columbus, OH
Guzior DV, Das A, Roy S, Sen CK. Non-invasive preclinical porcine maxillofacial model to study excessive scarring of the face following burn injury.
Denman Undergraduate Research Forum | April 2019 | Columbus, OH
Awards & Fellowships
Dissertation Completion Fellowship, The Graduate School, Michigan State University, 2024
Graduate School Travel Fellowship, The Graduate School, Michigan State University, 2023
Ralph Evans Award, Department of Microbiology, Genetics, & Immunology, Michigan State University, 2023
Outstanding Scholar Fellowship, College of Natural Science, Michigan State University, 2022-2023
Philipp and Vera Gerhardt Award, Department of Microbiology, Genetics, & Immunology, Michigan State University, 2021
Professional Memberships
Metabolomics Association of North America
American Society for Microbiology
Microbiology Society
Anaerobe Society of the Americas
Service & Leadership
Annual Fundraiser and Shavee (over $8,000 raised), St. Baldrick’s Foundation, 2015-Present
Graduate Student Mentor, Graduate Recruitment Initiative Team, Michigan State University, 2022-2024
Graduate Committee Representative, Department of Microbiology, Genetics, & Immunology, Michigan State University, 2022-2024
Volunteer, Science Debate - MSU Chapter, 2020
Donor, Be The Match, 2020
Skills & Expertise
Laboratory: targeted and untargeted metabolomics, liquid chromatography-tandem mass spectrometry, microbiome analysis, 16S amplicon sequencing, bacteriology, aerobic and anaerobic, biofilm culture, primer design, polymerase chain reaction (PCR), Gibson assembly, plasmid assembly and transformation, tissue sectioning, frozen or wax-embedded, immunohistochemistry, confocal and light microscopy, ZEN slide imaging (Zeiss Axio Scan Z1), wound harmonic ultrasound imaging, laser speckle microperfusion imaging, general photography, digital planimetry, troubleshooting, problem-solving
Computation and Statistics: high-performance computing, machine learning, hierarchical cluster analysis, statistics best practices, Adobe Illustrator, data visualization
Software: R, Python, QIIME 2, XCaliber, MZmine, Cytoscape, Adobe Illustrator, Microsoft 365
Communication: technical writing, peer-reviewed publication, public speaking, teaching, mentorship, fostering departmental, national, and international collaboration, active listening, empathy