Determining the impact of host-synovial fluid factors on Staphylococcus aureus aggregation
Published in The Ohio State University, 2019
Investigating how host factors within synovial fluid contribute to S. aureus aggregation in vitro. Read more
Posts by Collection
news
portfolio
publications
Investigation of synovial fluid induced Staphylococcus aureus aggregate development and its impact on surface attachment and biofilm formation
Published in PLoS ONE, 2020
This paper describes how various components found within synovial fluid, the biological lubricant present in joints, can impact aggregation and surface attachment during infection. Read more
Recommended citation: Pestrak MJ, Gupta TT, Dusane DH, Guzior DV, Staats A, Harro J, Horswill AR, Stoodley P. 2020. Investigation of synovial fluid induced *Staphylococcus aureus* aggregate development and its impact on surface attachment and biofilm formation. PLOS One 15(4):e0231791. https://doi.org/10.1371/journal.pone.0231791
Review: microbial transformations of human bile acids.
Published in Microbiome, 2021
This review summarizes recent literature known about microbial bile acid transformations in addition to discussing a novel fifth transformation, microbial bile acid conjugation. Read more
Recommended citation: Guzior DV, Quinn RA. 2021. Review: microbial transformations of human bile acids. Microbiome 9(1):140. https://doi.org/10.1186/s40168-021-01101-1
A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung
Published in Journal of Cystic Fibrosis, 2021
The first description of how Elexacaftor-Tezacaftor-Ivacaftor, also known as TRIKAFTA, therapy alters the lung microbiome community structure of patients with cystic fibrosis. Read more
Recommended citation: Sosinski LM, Martin C, Neugebauer KA, Ghuneim L-AJ, Guzior DV, Castillo-Bahena A, Mielke J, Thomas R, McClelland M, Conrad D, Quinn RA. 2021. A restructuring of microbiome niche space is associated with Elexacaftor-Tezacaftor-Ivacaftor therapy in the cystic fibrosis lung. J Cyst Fibros 21(6):996-1005. https://doi.org/10.1016/j.jcf.2021.11.003
Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis
Published in Cell Reports, 2021
Further understanding the impacts of antibiotics on the cecal microbiome and metabolome in the context of hyperglycemia. Read more
Recommended citation: Wurster JI, Peterson RL, Brown CE, Penumutchu S, Guzior DV, Neugebauer K, Sano WH, Sebastian MM, Quinn RA, Belenky P. 2021. Streptozotocin-induced hyperglycemia alters the cecal metabolome and exacerbates antibiotic-induced dysbiosis. Cell Rep 37(11):110113. https://doi.org/10.1016/j.celrep.2021.110113
Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection
Published in The ISME Journal, 2022
Changes in the cystic fibrosis lung can be predicted utilizing in silico models and further validated with experiments in vitro. Read more
Recommended citation: Ghuneim LAJ, Raghuvanshi R, Neugebauer KA, Guzior DV, Martin C, Schena B, Feiner JM, Castillo-Bahena A, Mielke J, McClelland M, Conrad D, Klapper I, Zhang T, Quinn RA. 2022. Complex and unexpected outcomes of antibiotic therapy against a polymicrobial infection. ISME J 16(9):2065–2075. https://doi.org/10.1038/s41396-022-01252-5
Baat gene knockout alters post-natal development, the gut microbiome, and reveals unusual bile acids in mice
Published in Journal of Lipid Research, 2022
Bile acid conjugation is important for early life development of mice, as knocking out the gene responsible for canonical murine bile acid conjugation results in significant physiological deficits early in life. Read more
Recommended citation: Neugebauer KA, Okros M, Guzior DV, Feiner J, Chargo NJ, Rzepka M, Schilmiller AL, O’Reilly S, Jones AD, Watson VE, Luyendyk JP, McCabe L, Quinn RA. 2022. Baat gene knockout alters post-natal development, the gut microbiome, and reveals unusual bile acids in mice. J Lipid Res 63(12):100297. https://doi.org/10.1016/j.jlr.2022.100297
A multi-omic trip through the human gut
Published in Nature Metabolism, 2023
Most of human microbiome research has focused on analysing faecal samples, which represent the final stop of the digestive journey. Two recent articles use a novel sampling approach to capture luminal content at different points during digestion and reveal that the analysis of faecal samples tells only a fraction of the story. Read more
Recommended citation: Quinn RA, Martin C, Guzior DV. 2023. A multi-omic trip through the human gut. Nat Metab 5(5): 720–721. https://doi.org/10.1038/s42255-023-00773-3
Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy
Published in Respiratory Research, 2023
Investigation into prolonged effects of ETI therapy on patients with cystic fibroses showed enhanced lung function and more varied lung microbiomes over time, even though colonization with CF pathogens are still prevalent. Read more
Recommended citation: Martin C, Guzior DV, Gonzalez CT, Okros M, Mielke J, Padillo L, Thomas R, McClelland M, Conrad D, Widder S, Quinn RA. 2023. Longitudinal microbial and molecular dynamics in the cystic fibrosis lung after Elexacaftor-Tezacaftor-Ivacaftor therapy. Respir. Res. 24:317. https://doi.org/10.1186/s12931-023-02630-z
Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice
Published in Frontiers in Cell and Developmental Biology, 2024
Dietary prune supplementation, even at 5%, can mitigate glucocorticoid-induced bone loss and shift gut microbiota in a murine model. Greater supplementation significantly enhanced bone density and influenced gut microbiota changes associated with improved bone health in mice. Read more
Recommended citation: Chargo NJ, Neugebauer K, Guzior DV, Quinn RA, Parameswaran N, McCabe LR. 2024. Glucocorticoid-induced osteoporosis is prevented by dietary prune in female mice. Front. Cell Dev. Biol. 11:1324649. https://doi.org/10.3389/fcell.2023.1324649
Bile salt hydrolase acyltransferase activity expands bile acid diversity
Published in Nature, 2024
Bile salt hydrolase, an enzyme with decades of research behind it, is identified as one of the culprits behind microbial bile acid conjugation, diversifying the bile acid pool and further establishing connections between microbially conjugated bile acids and gastrointestinal physiology. Read more
Recommended citation: Guzior DV, Okros M, Shivel M, Armwald B, Bridges C, Fu Y, Martin C, Schilmiller A, Miller WM, Ziegler KM, Sims MD, Maddens ME, Graham SF, Hausinger RP, Quinn RA. 2024. Bile salt hydrolase acyltransferase activity expands bile acid diversity. Nature 626:852-858. https://doi.org/10.1038/s41586-024-07017-8
Shifts in the functional capacity and metabolite composition of the gut microbiome during recovery from enteric infection
Published in Frontiers in Cellular and Infection Microbiology, 2024
Investigating the impact of enteric infection on the gut microbiome uncovers higher metabolic capacity during infection, yet greater chemical diversity afterwards. Read more
Recommended citation: Hansen ZA, Schilmiller AL, Guzior DV, Rudrik JT, Quinn RA, Vasco KA and Manning SD. 2024. Shifts in the functional capacity and metabolite composition of the gut microbiome during recovery from enteric infection. Front Cell Infect Microbiol 14:1359576. https://doi.org/10.3389/fcimb.2024.1359576
Longitudinal analyses of infants’ microbiome and metabolome reveal microbes and metabolites with seemingly coordinated dynamics
Published in Communications Biology, 2024
Uncovering the intricate connections between the development of the infant gut microbiome and corresponding shifts in fecal metabolite profiles, revealing dynamic interplay that may influence infant health. Read more
Recommended citation: Wu H, Guzior DV, Martin C, Neugebauer KA, Rzepka MM, Lumeng JC, Quinn RA, de los Campos G. 2024. Longitudinal analyses of infants microbiome and metabolome reveal microbes and metabolites with seemingly coordinated dynamics. Commun Biol 7:1506. https://doi.org/10.1038/s42003-024-07015-6
Host metabolism balances microbial regulation of bile acid signalling
Published in Nature, 2025
A family of host-produced bile acid-methylcysteamine conjugates, made by the intestinal enzyme VNN1, act as potent FXR antagonists that counterbalance the signalling of microbiota-derived free bile acids. Read more
Recommended citation: Won TH, Arifuzzaman M, Parkhurst CN, Miranda IC, Zhang B, Hu E, Kashyap S, Letourneau J, Jin WB, Fu Y, Guzior DV, Quinn RA, Guo CJ, David LA, Artis D, Schroeder FC. 2025. Host metabolism balances microbial regulation of bile acid signalling. Nature 638(8049):216–224. https://doi.org/10.1038/s41586-024-08379-9
Balance between bile acid conjugation and hydrolysis activity can alter outcomes of gut inflammation
Published in Nature Communications, 2025
Host-conjugated bile acids protect against colitis in Baat knockout mice, but their benefit depends on a delicate balance with microbial bile salt hydrolase activity, with specific bsh genes enriched in people with Crohn’s disease. Read more
Recommended citation: Fu Y, Guzior DV, Okros M, Bridges C, Rosset SL, González CT, Martin C, Karunarathne H, Watson VE, Quinn RA. 2025. Balance between bile acid conjugation and hydrolysis activity can alter outcomes of gut inflammation. Nat Commun 16:3434. https://doi.org/10.1038/s41467-025-58649-x
Microenvironmental effects of a non-antibiotic therapy for a chronic polymicrobial infection alter microbial physiology, competition, and virulence
Published in The ISME Journal, 2025
Modeling the effects of Elexacaftor-Tezacaftor-Ivacaftor therapy on airway mucus shows that reduced nutrient availability dampens Pseudomonas aeruginosa virulence factor production, reducing competition and toxicity in cystic fibrosis lung communities. Read more
Recommended citation: González CT, Martin C, Crane M, Gutierrez K, Thomas J, Remisoski L, Okros M, Fu Y, Guzior DV, Finkhouse D, Bridges C, Mielke J, Querido G, Padillo L, Girgis R, McClelland M, Conrad D, Li X, Quinn RA. 2025. Microenvironmental effects of a non-antibiotic therapy for a chronic polymicrobial infection alter microbial physiology, competition, and virulence. ISME J 19:wraf125. https://doi.org/10.1093/ismejo/wraf125
Short-term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice
Published in Physiological Reports, 2025
Just 10 days of methionine restriction, even while maintaining a high fat diet, rapidly reverses metabolic dysfunction in mice and produces distinct proteomic and metabolomic signatures across adipose tissue, liver, aorta, and plasma. Read more
Recommended citation: McGilvrey MI, Fortier B, Cooke D, Mahdi MA, Tero B, Potts CM, Kaija A, Ryzhova L, Cora C, Richardson A, Guzior DV, Pinz I, Vary C, Koza RA, Ables G, Liaw L. 2025. Short-term dietary methionine restriction with high fat diet counteracts metabolic dysfunction in male mice. Physiol Rep 13(12):e70405. https://doi.org/10.14814/phy2.70405
Serum cholic acid and cecal Faecalibaculum increase in a male-specific manner in a murine hepatocellular carcinoma model
Published in Journal of Lipid Research, 2025
In a bile acid excess model of hepatocellular carcinoma, tumor-bearing males accumulate free cholic acid in serum alongside sex-specific gut microbiota shifts, with unconjugated cholic acid tracking the abundance of Faecalibaculum. Read more
Recommended citation: Dean AE, Guzior DV, Quinn RA, Gaulke CA, Anakk S. 2025. Serum cholic acid and cecal *Faecalibaculum* increase in a male-specific manner in a murine hepatocellular carcinoma model. J Lipid Res 67(1):100954. https://doi.org/10.1016/j.jlr.2025.100954
Evexomostat (SDX-7320), a methionine aminopeptidase type 2 inhibitor, stimulates weight loss and inhibits obesity-accelerated tumor growth
Published in Frontiers in Oncology, 2026
The polymer-conjugated METAP2 inhibitor SDX-7320 promotes weight loss, improves insulin sensitivity, and attenuates obesity-accelerated tumor growth in mice, outperforming tirzepatide in tumor growth inhibition despite causing less weight loss. Read more
Recommended citation: Cornelius P, Mayes BA, Dannenberg AJ, Dufour PJ, Little S, Guzior DV, Petersen JS, Shanahan JM, Carver BJ. 2026. Evexomostat (SDX-7320), a methionine aminopeptidase type 2 inhibitor, stimulates weight loss and inhibits obesity-accelerated tumor growth. Front Oncol 16:1751681. https://doi.org/10.3389/fonc.2026.1751681
talks
Reversing Dogma: Bile Salt Hydrolase
Published:
Bile acids are key molecules involved in the digestion of fats and other nutrients. In this talk, I discuss the breadth of microorganisms capable of performing a recently discovered bile acid transformation, microbial conjugation, in addition to the impact of these novel bile acids on microbial growth. Read more
Bile salt hydrolase aminoacyltransferase activity from gut anaerobes expands the human bile acid pool
Published:
In this talk, I discuss how anaerobic bacteria within our gut are capable of ligating amino acids to bile acids via the enzyme bile salt hydrolase, pH dependence of this activity, and important active site residues. Read more
Developmental dynamics of the infant fecal metabolome
Published:
Investigating the correlation between early life exposures and subsequent health outcomes is crucial for the early treatment of various diseases. Here, I introduce an innovative approach to analyze both the metabolome and microbiome data from infant fecal samples. This method demonstrates how specific microbial taxa can induce changes in the chemical composition over time. Read more
teaching
Introduction to Microbiology, MMG301
Undergraduate Course, Michigan State University, Department of Microbiology and Molecular Genetics, 2020
Details
- MMG 301: Introduction to Microbiology
- Profs: Dr. Philip Delekta (MMG), Dr. Neal Hammer (MMG)
- Place: Online, via Zoom
Introductory Microbiology Lab, MMG302
Undergraduate Course, Michigan State University, Department of Microbiology and Molecular Genetics, 2020
Details
- MMG 302: Introductory Microbiology Lab
- Profs: Dr. Dennis Arvidson (MMG), Dr. Poorna Viswanathan (MMG)
- Place: Online, via Zoom